ALDOA Sheep Polyclonal Antibody

CAT#: AP20105PU-N

ALDOA sheep polyclonal antibody, Purified

Conjugation: Unconjugated Biotin



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CNY 7,710.00


货期*
5周

规格
    • 1 mg

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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommend Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques, to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Working Dilutions:
Non-precipitating antibody-binding techniques: 1/100-1/5,000.
Reactivity Rabbit
Host Sheep
Clonality Polyclonal
Immunogen Aldolase isolated and purified from Rabbit muscle.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore by adding 0.5 ml of sterile distilled water
Concentration lot specific
Purification Solid Phase Affinity Chromatography
Conjugation Unconjugated
Storage Condition Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Background This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
Synonyms ALDA, NY-LU-1, Muscle-type aldolase
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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