MELK Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of MELK protein. (region surrounding asn449)
Formulation	PBS with 0.02% sodium azide, 50% glycerol, pH7.2  State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~75 kDa
Gene Name	maternal embryonic leucine zipper kinase
Database Link	Entrez Gene 9833 Human UniProt ID Q14680
Background	Maternal Embryonic Leucine Zipper Kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain and a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNAbinding proteins and is presumed to be involved in protein-DNA interactions, and potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg and early embryo. Mouse and human MELK proteins share 95% sequence identity in the kinase domain and northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis and to oocytes in the ovary.
Synonyms	Maternal embryonic leucine zipper kinase, KIAA0175, hMELK
Reference Data
Protein Families	Druggable Genome, Protein Kinase

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