PAX1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of Pax-1 protein. (region surrounding Thr367)
Formulation	Phosphate buffered saline (PBS), pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 36 kDa
Gene Name	paired box 1
Database Link	Entrez Gene 5075 Human UniProt ID P15863
Background	Pax genes contain paired domains with strong homology to genes in Drosophila that are involved in programming early development. Pax-1 is a sequence-specific DNA binding-protein with transcriptional activating properties. The expression pattern of Pax-1 during mouse embryogenesis indicates that it may play an important role in the development of the vertebral column. The autosomal recessive mutation ‘undulated’ (un) in the mouse exhibits vertebral anomalies along the entire rostrocaudal axis and is associated with a point mutation (G-to-A transition) at position 15 leading to a gly-to-ser replacement in a highly conserved region of the paired box of Pax-1. Pax-1 is required for the normal development of these three skeletal elements: the vertebral column, sternum, and scapula. Mice who are doubly heterozygous for the mutants ‘undulated’ and ‘Patch’ have a phenotype reminiscent of an extreme form of spina bifida occulta in humans. The gene which encodes Pax-1 maps to human chromosome 20p11.2.
Synonyms	Pax-1, HUP48
Reference Data

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