Collagenase Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). Working Dilutions in non-precipitating antibody-binding techniques: 1/5,000-1/100,000.
Reactivity	Clostridium histolyticum
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagenase is isolated and purified from Clostridium histolyticum (containing the different isoforms). Freund’s complete adjuvant is used in the first step of the immunization
Specificity	Collagenase from Clostridium histolyticum (containing the different isoforms). The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized Hyperimmune IgG fraction Molar radio: Biotin/ IgG ~2.2
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term.  If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated freezing and thawing.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.