ETFA (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.05-0.2 µg/ml. This antibody detects a band of ~30kDa in Human, Mouse and Rat Heart lysates.
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C Terminus of the protein sequence according to NP_000117.1 and NP_001121188.1.
Specificity	This antibody is expected to recognize both reported isoforms (NP_000117.1 and NP_001121188.1).
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	electron transfer flavoprotein alpha subunit
Database Link	Entrez Gene 110842 MouseEntrez Gene 300726 RatEntrez Gene 2108 Human UniProt ID P13804
Background	ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
Synonyms	Alpha-ETF
Note	Calculated Molecular Weight: 30.0kDa (NP_001121188.1).
Reference Data
Protein Families	Druggable Genome

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