LOC102163346 Rabbit Polyclonal Antibody
CAT#: AP21345AF-N
LOC102163346 rabbit polyclonal antibody, Azide Free
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CNY 3,402.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, ID, IF, IP, R, WB |
Recommend Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice. Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/40,000. |
Reactivity | Porcine |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Malic dehydrogenase is isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Formulation | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized IgG fraction |
Reconstitution Method | Restore by adding 1.0 ml sterile distilled water |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
Database Link | |
Background | Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase. |
Synonyms | MDHA, Malate dehydrogenase, cytoplasmic |
Reference Data |
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