LDHB Rabbit Polyclonal Antibody
CAT#: AP21409PU-N
LDHB rabbit polyclonal antibody, Aff - Purified
Conjugation: Biotin
Need it in bulk or conjugated?
Get a free quote
CNY 7,161.00
货期*
5周
规格
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Applications | ELISA, ID, IF, IP, R, WB |
Recommend Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Recommended Dilutions: Non-precipitating antibody-binding techniques: 1/100-1/2,000. |
Reactivity | Porcine |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | L-Lactic Dehydrogenase isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | L-Lactic Dehydrogenase from Porcine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Formulation | PBS, pH 7.2 stabilized with Dextran containig no preservatives or foreign proteins State: Aff - Purified State: Lyophilized purified hyperimmune IgG fraction |
Reconstitution Method | Restore by adding 0.5 ml of sterile distilled water |
Concentration | lot specific |
Purification | Solid Phase Affinity Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
Database Link | |
Background | Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream). |
Synonyms | LDHB, L-lactate dehydrogenase B chain, LDH-B, EC=1.1.1.27, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46 |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |
Customer
Reviews
Loading...