Complement C5 (C5) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IHC, IP
Recommend Dilution	The fluorescent immunoconjugate to Human C5 is used to determine the presence and pattern using Immunohistochemical staining techniques. Determinations of individual C components can be very useful in defining the exact location of a defect. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: 1/10-1/40.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	C5 protein isolated and purified from pooled normal human serum. Freund’s complete adjuvant is used in the first step of the immunization
Specificity	The reactivity of the antiserum is restricted to C5. In immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum. The antiserum does not cross react with any other component of human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation	PBS, pH 7.2 without preservatives. Label: FITC State: Lyophilized purified Hyperimmune IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1 Absorption emission: 492 nm / 515 nm Molar radio: Fluorescein/IgG ~2.2
Reconstitution Method	Restore by adding 1 ml of sterile distilled water.
Concentration	lot specific
Purification	The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
Conjugation	FITC
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one week or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	complement component 5
Database Link	Entrez Gene 727 Human UniProt ID P01031
Background	C5 component of human complement is a plasma protein consisting of two polypeptide chains, alpha, beta, bound by disulphide bonds. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack complex.
Synonyms	CPAMD4, Complement component 5
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus

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