CIRBP (81-91) Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, PEP-ELISA, WB
Recommend Dilution	Peptide ELISA: Detection limit: 1/16000. Western blot: 0.05-0.2 µg/ml. Approx 18kDa band observed in Mouse and Rat Testis lysates. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunizing peptide.
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_001271.1.
Specificity	Recognizes CIRBP (aa 81-91).
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cold inducible RNA binding protein
Database Link	Entrez Gene 1153 Human UniProt ID Q14011
Background	CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation. It acts as a translational repressor. CIRBP is an immortalized gene in murine primary cells. CIRBP promotes assembly of stress granules (SGs), when overexpressed. It is a potential proto-oncogenic proteins upon overexpression. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature.
Synonyms	A18HNRNP, CIRP
Note	Calculated Molecular Weight: 18.6kDa (Mouse NP_031731.1 and Rat NP_112409.2).
Reference Data

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