Isocitrate dehydrogenase (IDH1) (65-77) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: 1/1000 (Detection Limit). Western blot: 0.1-0.3 µg/ml. Approx 40+45kDa bands observed in lysates of cell line HepG2 and 40kDa in Mouse and Rat Liver lysates. The observed 40kDa is consistent with observaton with different antibodies from other commerical sources.
Reactivity	Bovine, Canine, Human, Mouse, Rat, Sheep
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_005887.2.
Specificity	Recognizes IDH1 (65-77).
Formulation	Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	isocitrate dehydrogenase (NADP(+)) 1, cytosolic
Database Link	Entrez Gene 3417 Human UniProt ID O75874
Background	IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Synonyms	PICD, Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, Oxalosuccinate decarboxylase
Note	Calculated Molecular Weight: 46.7kDa (NP_005887.2).
Reference Data

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