APOBEC3G (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/16000. Immunohistochemistry on Paraffin Sections: 2.5 µg/ml. Western Blot: 0.5 - 1.5 µg/ml.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-Terminus of Human APOBEC3G (NP_068594.1). Percent identity by BLAST analysis: Human, Baboon (100%); Monkey (92%); Chimpanzee, Gorilla, Gibbon, Marmoset (83%).
Specificity	This antibody detects APOBEC3G (C-term).
Formulation	Tris saline, pH~7.3 State: Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
Gene Name	apolipoprotein B mRNA editing enzyme catalytic subunit 3G
Database Link	Entrez Gene 60489 Human UniProt ID Q9HC16
Background	The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5a which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.
Synonyms	ARP9, CEM15, MDS019, APOBEC-3G, ARCD
Reference Data

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