GAPDH (9-323) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry: 1/100 - 1/200. Immunofluorescence: 1/100 - 1/200. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 1/5000 - 1/20000.
Reactivity	Bovine, Canine, Drosophila, Feline, Human, Mouse, Rabbit, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein fragment corresponding to a region within amino acids 9 and 323 of GAPDH
Specificity	This antibody detects GAPDH at aa 9-323.
Formulation	PBS, 20% glycerol (pH 7), 0.025% proclin State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store (in aliquots) at -20 °C. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human UniProt ID P04406
Background	GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Synonyms	GAPD, CDABP0047
Note	The antibody is predicted to cross react with these species based on the following homology: Rat (94%), Rabbit (94%), cow (94%), Dog (95%), Cat (96%).
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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