MIF (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect MIF in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect MIF in formalin fixed and paraffin embedded tissues.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence at the C-terminal of human MIF
Specificity	This antibody detects MIF at C-term. No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Database Link	Entrez Gene 17319 MouseEntrez Gene 81683 RatEntrez Gene 4282 Human UniProt ID P14174
Background	Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages,and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2 MIF plays a critical role in inflammatory diseases and atherogenesis.
Synonyms	Macrophage migration inhibitory factor, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF, MMIF
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Phenylalanine metabolism, Tyrosine metabolism

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