alpha 2a Adrenergic Receptor (ADRA2A) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 0.1-0.5 μg/ml with the appropriate system to detect ADRA2 in cells and tissues. Immunohistochemistry on Paraffin Sections: 0.5-1 μg/ml to detect ADRA2 in formalin fixed and paraffin embedded tissues. Boiling the sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Reactivity	Human, Mouse, Rabbit, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence mapping near the C-terminal of Human ADRA2 (aa 414-431)
Specificity	This antibody detects Alpha-2A adrenergic receptor near C-term. No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium Azide State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 0.2ml of distilled water to yield a concentration of 0.5 mg/ml.
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	adrenoceptor alpha 2A
Database Link	Entrez Gene 11551 MouseEntrez Gene 25083 RatEntrez Gene 150 Human UniProt ID P08913
Background	Alpha-2-adrenergic receptor (ADRA2), also known as platelet type adrenoceptor alpha-2A ,is a member of G protein-coupled receptor superfamily. This gene, which can identify a DraI RFLP of the ADRAR gene, mapped to 10q23-q25, is found in the distal region of mouse chromosome 19 and abundantly expressed in giant cell. ADRA2 acts a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. It has 3 highly homologous subtypes: ADRA2A; ADRA2B and ADRA2C. Studies in mouse suggested that both the ADRA2A and ADRA2C subtypes are required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. ADRA2A receptors inhibited transmitter release at high stimulation frequencies, whereas the ADRA2C subtype modulated neurotransmission at lower levels of nerve activity.
Synonyms	ADRA2A, ADRA2R, ADRAR, Alpha-2A adrenoreceptor, Alpha-2A adrenoceptor
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.