Sonic Hedgehog (SHH) (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect SHH in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect SHH in formalin fixed and paraffin embedded tissues. Immunocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to a sequence near the N-terminal of human SHH
Specificity	This antibody detects Sonic hedgehog (SHH) near N-term. No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	sonic hedgehog
Database Link	Entrez Gene 20423 MouseEntrez Gene 29499 RatEntrez Gene 6469 Human UniProt ID Q15465
Background	The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined; however, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.
Synonyms	Sonic hedgehog protein, HHG-1
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways	Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

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