PP2A-alpha (PPP2CA) (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect PP2A in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect PP2A in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry: At 0.5-1μg/ml to detect PP2A in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence mapping near the N-terminal of human PP2A
Specificity	This antibody detects PPP2CA at N-term. No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	protein phosphatase 2 catalytic subunit alpha
Database Link	Entrez Gene 19052 MouseEntrez Gene 24672 RatEntrez Gene 5515 Human UniProt ID P67775
Background	The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
Synonyms	Protein Phospatase 2A, PP2A-alpha, Replication protein C
Reference Data
Protein Families	Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways	Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway

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