SKP2 (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: At 1μg/ml with the appropriate system to detect SKP2 in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect SKP2 in formalin fixed and paraffin embedded tissues. Immunocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	synthetic peptide corresponding to a sequence at the N-terminal of human SKP2
Specificity	This antibody detects SKP2 / FBXL1 (N-term). No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
Database Link	Entrez Gene 6502 Human UniProt ID Q13309
Background	The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.
Synonyms	FBL1; FBXL1; FLB1; MGC1366; p45skp2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Acute myeloid leukemia, Apoptosis, Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Small cell lung cancer, Ubiquitin mediated proteolysis

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