DNase I (DNASE1) (1-252) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry: 1/100 - 1/1000. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 1/500 - 1/3000.
Reactivity	Canine, Human, Rabbit
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 252 of DNase I.
Specificity	This antibody recognizes Deoxyribonuclease I (DNASE1) at aa 1-252.
Formulation	0.1 M Tris-glycine, pH 7.0 containing 10% Glycerol and 0.01% Thimerosal State: Aff - Purified State: Liquid purfied Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	deoxyribonuclease I
Database Link	Entrez Gene 1773 Human UniProt ID P24855
Background	Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
Synonyms	DNASE1, DNL1, DRNI, Deoxyribonuclease I, DNase I
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane

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