Tryptophan 5 hydroxylase 2 (TPH2) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: Detection Limit: 1/16000. Western Blot: Preliminary experiments in human brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). Immunohistochemistry on Paraffin Sections: 4-6 µg/ml.  In Paraffin Embedded Human Brain Stem shows layered neuronal staining.
Reactivity	Canine, Human, Bovine, Porcine
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_775489.2.
Specificity	Recognizes Tryptophan hydroxylase 2 / TPH2
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tryptophan hydroxylase 2
Database Link	Entrez Gene 121278 Human UniProt ID Q8IWU9
Background	Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).
Synonyms	Tryptophan 5-monooxygenase 2, Neuronal tryptophan hydroxylase, NTPH
Reference Data

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