B7-1 (CD80) Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, PEP-ELISA, WB
Recommend Dilution	Peptide ELISA: Detection Limit: 1/4000. Western blot: 0.3-1 µg/ml. Detects a band of Approx 60kDa band observed in Human Lymph Node and Human Tonsil lysates (calculated MW of 33.0kDa according to NP_005182.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_005182.1.
Specificity	Recognizes CD80.
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD80 molecule
Database Link	Entrez Gene 941 Human UniProt ID P33681
Background	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. Cell Distribution: CD80 is rapidly induced on the surface of activated B cells, T cells and monocytes and is also expressed at high levels on dendritic cells.
Synonyms	CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.