Ikaros (IKZF1) Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC, PEP-ELISA
Recommend Dilution	Peptide ELISA: 1/1000. Western blot: Approx 65kDa band observed in lysates of cell line MOLT4 (calculated MW of 57.5kDa according to NP_006051.1). Recommended concentration: 1-3 µg/ml. Similar results were obtained with AP17016PU-N, which was developed against a different part of the protein. Immunohistochemistry on Paraffin Sections: 2.5-3.8 µg/ml. In paraffin embedded Human Tonsil shows textured staining in some nuclei of the lymphoid tissue.
Reactivity	Bovine, Canine, Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_006051.1.
Specificity	This antibody recognizes IKAROS.
Formulation	Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	IKAROS family zinc finger 1
Database Link	Entrez Gene 10320 Human UniProt ID Q13422
Background	DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.
Synonyms	IK1, LYF-1, LYF1, ZNFN1A1
Reference Data

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