TRIM29 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	Peptide ELISA: 1/128000 (Detection Limit). Western blot: Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8kDa according to NP_036233.2 (isoform 1). Primary incubation 1 hour at room tempetature. Recommended concentration: 0.1-1 µg/ml. Immunohistochemistry on Paraffin Sections: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 1-2 µg/ml. Flow Cytometry analysis of A431 cells. Recommended concentration: 10 µg/ml.
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_036233.2.
Specificity	This antibody recognizes TRIM29.
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tripartite motif containing 29
Database Link	Entrez Gene 23650 Human UniProt ID Q14134
Background	Ataxia-telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.
Synonyms	ATDC; FLJ36085
Reference Data

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