Csf2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, Neutralize, WB
Recommend Dilution	Neutralization. To yield one-half maximal inhibition [ND50] of the biological activity of Rat GM-CSF (0.5 ng/ml), a concentration of 0.09–0.14 μg/ml of this antibody is required. Sandwich ELISA: To detect Rat GM-CSF by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat GM-CSF (AP23790BT-N or AP23790BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat GM-CSF. Western Blot: To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure recombinant Rat GM-CSF.
Specificity	Recognizes Rat GM-CSF. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing an immobilized Rat GM-CSF matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	colony stimulating factor 2
Database Link	Entrez Gene 116630 Rat UniProt ID P48750
Background	The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.
Synonyms	CSF2, GMCSF, Sargramostim, Molgramostin
Reference Data

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