PDCD10 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western Blot: 1-5 μg/ml. Immunofluorescence: 2-10 µg/ml. Immunohistochemistry.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 95%) recombinant Human CCM3, amino acids Met1-Ala212 derived from E.coli (Cat.-No AR26004PU-N)
Specificity	This antibody will detect recombinant Human CCM-3 in Western Blot and native CCM-3 in Immunohistochemistry.
Formulation	5mM PBS, pH 7.2 without preservatives or stabilizers State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography with Immobilized Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	programmed cell death 10
Database Link	Entrez Gene 11235 Human UniProt ID Q9BUL8
Background	Cerebral cavernous malformations (CCMs) are sporadically acquired or inherited vascular lesions of the central nervous system consisting of clusters of dilated thin-walled blood vessels that predispose individuals to seizures and stroke. Mutations in CCM1, CCM2, or CCM3 lead to cerebral cavernous malformations, one of the most common hereditary vascular diseases of the brain. Endothelial cells within these lesions are the main disease compartments. Here, we show that adenoviral CCM3 expression inhibits endothelial cell migration, proliferation, and tube formation while down regulation of endogenous CCM3 results in increased formation of tube-like structures. Adenoviral CCM3 expression does not induce apoptosis under normal endothelial cell culture conditions but protects endothelial cells from staurosporine-induced cell death. Tyrosine kinase activity profiling suggests that CCM3 supports PDPK-1/Akt-mediated endothelial cell quiescence and survival (Schleider et al, Neurogenetics 12, 2011). The CCM-3 is fused to a N-terminal His-tag (6x His).
Synonyms	CCM3, TFAR15
Reference Data
Protein Families	Druggable Genome

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