Snap25 pSer187 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/1000.
Reactivity	Guinea Pig, Human, Mouse, Primate, Rat, Zebrafish
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser187 of SNAP25
Specificity	Specific for the ~25k SNAP25 protein phosphorylated at Ser 187 in Western blots. Immunolabeling is completely blocked by blocked λ -Ptase.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Purification	Affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	synaptosomal-associated protein 25
Database Link	Entrez Gene 25012 Rat UniProt ID P60881
Background	SNAP25 (Synaptosomal associated pr otein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al., 1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on ser187 (Shu et al ., 2008). Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).
Synonyms	SNAP-25, SUP, SNAP, Super Protein, RIC4, ric-4
Reference Data

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