Grin2a (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IP, WB
Recommend Dilution	Western blot: 1/1000. Immunohistochemistry: 1/1000. Immunoprecipitation: 6 μl per 200 μg lysate.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein from the C-terminus of the NR2A subunit of rat NMDA receptor.
Specificity	Specific for the ~180k NR2A subunit of the NMDA receptor. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Purification	Affinity purification using a column to which the fusion protein immunogen was coupled
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	glutamate ionotropic receptor NMDA type subunit 2A
Database Link	Entrez Gene 24409 Rat UniProt ID Q00959
Background	The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K /AMPAR). Those that are sensitive to N-methyl-D- aspartate (NMDA) are designated NMDA receptors (N MDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been im plicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such cha nnels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Synonyms	NMDAR2A, GRIN2A, NR2A
Reference Data

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