RHOH (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/5000. Immunofluorescence: 1/100 - 1/500. Immunohistochemistry on Paraffin Sections: 1/200. Western Blot: 1/500 - 1/1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide - KLH conjugated
Specificity	This antibody detects endogenous levels of total RhoH protein.
Formulation	PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ras homolog family member H
Database Link	Entrez Gene 74734 MouseEntrez Gene 305341 RatEntrez Gene 399 Human UniProt ID Q15669
Background	The Rho subfamily of small GTP-binding proteins mediates many fundamental cellular functions. The commonly studied members (Rho, Rac, and CDC42) regulate actin reorganization and affect diverse cellular responses, including adhesion, cytokinesis, and motility. RhoH, also known as TTF (Translocation Three Four), Rho-related GTP-binding protein and ras homolog gene family member H, is unlike most other small G proteins. Most small G proteins are expressed ubiquitously, however, Rho H is expressed only in hemopoietic cells and tissues. Translocations and a high frequency of Rho H mutation have been detected in primary lymphoma cells. Rho H expression has also been observed in activated neutrophils. RhoH is GTPase deficient, remaining in a GTP-bound activated state without cycling. Rho H may be involved in the functional differentiation of T cells and in cytoskeleton organization. The RhoH/TTF (ARHH) gene maps to chromosome 4p13 and encodes a 191 -amino acid polypeptide.
Synonyms	ARHH, TTF
Reference Data
Protein Families	Transcription Factors
Protein Pathways	Leukocyte transendothelial migration

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