Rat Serum Proteins Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IHC, IP
Recommend Dilution	Can be used for direct fluorescent staining of rat serum proteins at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80.
Reactivity	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Pooled whole serum and selected protein fractions isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Tested in immunoelectrophoresis and double radial immunodiffusion against pooled serum precipitation of at least 12 individual proteins have been observed. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation	PBS, pH 7.2 No preservative added. No foreign proteins added. Label: FITC State: Lyophilised hyperimmune Ig fraction Label: Fluorescein isothiocyanate isomer 1 Absorption emission: 492nm / 515nm Molar radio: 1,8
Reconstitution Method	Restore with 1 ml sterile distilled water
Concentration	lot specific
Purification	DEAE-column Chromatography
Conjugation	FITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Reference Data

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