ALB Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, IP, R, WB
Recommend Dilution	Can be used as reagent for the direct detection of albumin in rabbit cells, tissues and body fluids in immunofluorescence techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80.
Reactivity	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Albumin is isolated from rabbit serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The defined antibody specificity is directed to albumin as tested against rabbit sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rabbit serum and the purified albumin. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been not been tested in detail.
Formulation	PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. Label: FITC State: Lyophilised hyperimmune Ig fraction Label: Fluorescein isothiocyanate isomer 1 Absorption emission: 492nm / 515nm Molar radio: 1,8
Reconstitution Method	Restore with 1 ml sterile distilled water
Concentration	lot specific
Purification	DEAE-column Chromatography
Conjugation	FITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 100009195 Rabbit UniProt ID P49065
Background	Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules.
Synonyms	ALB, BSA, HSA, Serum Albumin
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Reference Data

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