SFRS3 (SRSF3) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/5000. Immunofluorescence: 1/100 - 1/500. Immunohistochemistry on Paraffin Sections: 1/200. Western Blot: 1/500 - 1/1000.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from Human SFRS3.
Specificity	This antibody detects endogenous levels of total SFRS3 protein.
Formulation	PBS (without Mg2+, Ca2+), pH 7.4, 150 mM Sodium Chloride, 0.02% Sodium Azide, 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	serine/arginine-rich splicing factor 3
Database Link	Entrez Gene 20383 MouseEntrez Gene 6428 Human UniProt ID P84103
Background	Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine(RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and RNA.
Synonyms	SRP20
Reference Data
Protein Families	Stem cell - Pluripotency
Protein Pathways	Spliceosome

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