Pgf Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 2-5 μg/ml. No cross-reactivity with recombinant Human PlGF under non-reducing conditions is observed. Immunofluorescence/Immunohistochemistry: 1/200. Immunohistochemistry on Paraffin Sections.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 95%) recombinant Mouse PlGF (Ala24-Pro158) derived from Sf9 insect cells (Cat.-No AR26011PU-L).
Specificity	Recognizes Mouse PlGF. Other species not tested.
Formulation	5mM PBS, pH 7.2 Endotoxin level: < 0.1 EU/1 µg of the antibody (LAL) State: Purified State: Liquid purified IgG fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	placental growth factor
Database Link	Entrez Gene 18654 Mouse UniProt ID P49764
Background	Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF1, PlGF2 and PlGF3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF2 has been identified. Mouse PlGF shares 65% amino acid identity with human PlGF2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high affinity to Flt1, but not to Flk1/KDR.
Synonyms	PGFL, PLGF, PlGF
Reference Data

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