Choline Acetyltransferase (CHAT) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemstry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: The antibody was analyzed by Immunohistochemistry (at 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (Cat.-No AP31795FC-N, 1/500 dilution) as the secondary reagent.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Synthetic peptide KLH conjugated corresponding to a region of the Choline Acetyltransferase gene product shared between the Human (P28329) and Mouse (Q03059) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks and then affinity-purified using a peptide column.
Specificity	Recognizes Choline Acetyltransferase (ChAT).
Formulation	10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage Condition	Store undiluted in the dark at 2-8°C.
Gene Name	choline O-acetyltransferase
Database Link	Entrez Gene 12647 MouseEntrez Gene 1103 Human UniProt ID P28329
Background	Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
Synonyms	Choline O-acetyltransferase, Choline acetylase, CHOACTase, ChAT, EC=2.3.1.6
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Glycerophospholipid metabolism

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