CD11b (ITGAM) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/2000-1/5000. Quality Control: This antibody was analyzed by Immunohistochemistry (at 3 µg/ml) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) or HRP-labeled Goat anti-Chicken IgY (1/2000 dilution, Cat.-No AP31795HR-N) as the secondary reagent.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Synthetic peptide KLH-conjugated corresponding to a sequence shared between the Mouse (NP_001076429.1) and Human gene products (NP_001139280.1). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks.
Specificity	Recognizes Integrin Alpha-M (MAC-1, OX-42 Antigen).
Formulation	10mM PBS, pH 7.2 containing 50% Glycerol and 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted in the dark at 2-8°C.
Gene Name	integrin subunit alpha M
Database Link	Entrez Gene 16409 MouseEntrez Gene 3684 Human UniProt ID P11215
Background	Integrin Alpha M (also known as MAC-1 and CD11b) is a 1142 amino acid protein (MAC-1): expressed in various antigen-presenting cells of the immune system, including microglial cells of the central nervous system. This is the same protein that is recognized by the Ox-42 Mouse monoclonal antibody. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

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