Acpp (Pain System Marker) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunocytochemistry. Immunohistochemistry (1/500-1/1000). Quality Control: Antibodies were analyzed using immunohistochemistry with tissue sections through a 10%-formalin fixed adult Mouse. Sections were examined for PAP-positive dorsal root ganglion sensory neurons. Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) used as the secondary reagent.
Reactivity	Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system. Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.
Specificity	Recognizes Mouse Prostatic Acid Phosphatase (PAP).
Formulation	10mM PBS, pH 7.2 containing 1% BSA as stabilizer and 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted in the dark at 2-8°C.
Gene Name	acid phosphatase, prostate
Database Link	Entrez Gene 56318 Mouse UniProt ID Q8CE08
Background	Mouse PAP is a 43,698 dalton protein (381 amino acids; NCBI accession numberAAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.
Synonyms	Prostate acid phosphatase, PAP, ACP3, PSAP
Reference Data

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