Tau (MAPT) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/20000-1/50000 for Western Blots. 1/10000-1/20000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: Both of these anti-peptide antibodies were analyzed by Immunohistochemistry (at a dilution of 1/20000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Antipeptide antibodies were generated in chickens against two sequences shared between the Mouse (P10637.3) and Human (P10636.5) gene products. Neither of these sequences contain phosphorylated serine or threonine residues, so these tau antibodies recognize both the euphosphorylated and hyperphosphorylated forms of Tau. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Specificity	Recognizes Tau.
Formulation	10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction
Concentration	lot specific
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted in the dark at 2-8°C.
Gene Name	microtubule associated protein tau
Database Link	Entrez Gene 17762 MouseEntrez Gene 4137 Human UniProt ID P10636
Background	Human Tau protein (a.k.a., “microtubule-associated protein tau isoform 1”) is a 78,902 dalton protein (758 amino acids) expressed in cortical neurons of the CNS. In normal individuals, tau contains moderate degrees of phosphorylation on various serine and threonine residues scattered throughout the N-terminal half of this protein. However in various neurodegenerative diseases, such as Alzheimer’s disease, Tau becomes hyperphosphorylated on these residues, leading to the pathological formation of intracellular “neurofibrillary tangles.” Such tangles are hallmarks of Alzheimer’s disease, as well as various other neurodegenerative diseases, collectively known as the “tauopathies.”
Synonyms	MAPTL, MTBT1, Microtubule-associated protein tau, PHF-tau, Neurofibrillary tangle protein, Paired helical filament-tau
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, MAPK signaling pathway

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