Telomerase reverse transcriptase (TERT) (597-611) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: 0.3-1 µg/ml. Approx 140kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 127kDa according to NP_937983.2).
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_937983.2; NP_001180305.1.
Specificity	This antibody recognizes TERT (597-611). It is expected to recognize both reported isoforms (NP_937983.2, (NP_001180305.1).
Formulation	Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	telomerase reverse transcriptase
Database Link	Entrez Gene 7015 Human UniProt ID O14746
Background	Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.
Synonyms	Telomerase reverse transcriptase, HEST2, EST2, TCS1, TRT
Reference Data
Protein Families	Druggable Genome

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