Clu (312-325) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: 1/8000 (Detection Limit). Western blot: 0.1-0.3 µg/ml. Approx 37kDa band observed in Mouse Testis lysates (calculated MW of 51.7kDa according to NP_038520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104(12):1407-12.; PMID: 11560857).
Reactivity	Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_038520.2.
Specificity	This antibody recognizes Mouse Clusterin / ApoJ (312-325). Other species not tested.
Formulation	Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	clusterin
Database Link	Entrez Gene 12759 Mouse UniProt ID Q06890
Background	At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.
Synonyms	Clusterin, APOJ, CLI, KUB1, AAG4, NA1/NA2, SP-40,40
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.