MSH5 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/64000 (Detection Limit). Western blot: Preliminary experiments gave an approx 38kDa band in Human Testis, Tonsil and Thymus lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kDa according to the smallest reported isoform NP_002432.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry on Paraffin Sections: 2.5-3.8 µg/ml. In paraffin embedded Human Testis shows nuclear staining in spermatocytes.
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_079535.3, NP_751897.1, NP_002432.1.
Specificity	This antibody is expected to recognise three reported isoforms ( NP_079535.3, NP_751897.1 and NP_002432.1.
Formulation	Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mutS homolog 5
Database Link	Entrez Gene 17687 MouseEntrez Gene 294252 RatEntrez Gene 4439 Human UniProt ID O43196
Synonyms	MutS protein homolog 5
Reference Data
Protein Families	Druggable Genome

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