Granulin (GRN) (248-259) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: 1/8000 (Detection limit). Western blot: 1-3 µg/ml. detects a band of Approx 75kDa in lysates of cell lines A431, A549 and HeLa. The observed molecular weight corresponds to earlier findings in literature with different antibodies (He and Bateman, Cancer Res. 1999 Jul 1;59(13):3222-9. PMID: 10397269).
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal of the protein sequence according to NP_002078.1.
Specificity	This antibody is expected to recognize the C-Terminal part of Granulin-3.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	63.5 kDa (NP_002078.1).
Gene Name	granulin
Database Link	Entrez Gene 2896 Human UniProt ID P28799
Background	Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while Granulin-3 acts as an antagonist to Granulin-4, inhibiting the growth. Tissue specificity: In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. Involvement in disease: Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
Synonyms	GRN, Granulins, Proepithelin, PEPI
Reference Data

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