ZNF217 Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, IHC, PEP-ELISA
Recommend Dilution	Peptide ELISA: 1/16000 (Detection limit).  Western blot: Preliminary experiments gave an approx 75kDa band in Human Prostate, Spleen, Testis and Thymus lysates after 0.03 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_006517.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemitry on Paraffin Sections: 3-6 µg/ml. In paraffin embedded Human Prostate shows nuclear staning in select secretory cells.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide with sequence from the internal region of Human ZNF217 (NP_006517.1).
Specificity	This antibody recognizes ZNF217
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	zinc finger protein 217
Database Link	Entrez Gene 7764 Human UniProt ID O75362
Background	ZNF217 binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473',
Synonyms	Zinc finger protein 217, ZABC1
Reference Data

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