ATG4B (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide between 23~53 amino acids from the N-terminal region of Human APG4B
Specificity	This antibody recognizes Human and Mouse ATG4B (N-term). Other species not tested.
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	44309 Da
Gene Name	autophagy related 4B cysteine peptidase
Database Link	Entrez Gene 23192 Human UniProt ID Q9Y4P1
Background	Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Synonyms	Autophagin-1, AUTL1, KIAA0943
Reference Data

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