LYZ Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. Recommended Working Dilutions: Histochemical and cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Immunohistochemistry on Paraffin Sections: 1/400.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified lysozyme is isolated from pooled milk. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. Cross-reactivity: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation	PBS, pH 7.2 State: Azide Free State: Lyophilized Purified hyperimmune IgG fraction Stabilizer: None Preservative: None
Reconstitution Method	Restore by by adding 1 ml sterile di stilled water
Concentration	lot specific
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt precipitation and purification of the IgG fraction by DEAE-chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	lysozyme (renal amyloidosis)
Database Link	Entrez Gene 396218 Chicken UniProt ID P00698
Background	Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low; in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.
Synonyms	LYZ, LZM
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Reference Data

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