Doublecortin (DCX) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunofluorescence/Immnunocytochemistry. Immunohistochemistry. Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at a concentration of 2 µg/ml) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Formulation	Sodium Phosphate-Buffered (10 mM) isotonic (0.9%, w/v) Saline (PBS, pH 7.2) State: Purified State: Liquid purified IgY fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. This product is photosensitive and should be protected from light.
Gene Name	doublecortin
Database Link	Entrez Gene 1641 Human UniProt ID O43602
Synonyms	Lissencephalin-X, Doublin, DCX, DBCN, LISX
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.