GAP43 (C-term) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Quality Control: Quality assurance analysis was performed using Immunohistochemistry (at 1/1000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/1000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemstry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells and 1/2000-1/5000 for western blots.
Reactivity	Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized.
Specificity	Specific for the ~43k GAP-43 protein.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Liquid purified IgY fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	growth associated protein 43
Database Link	UniProt ID P17677
Background	GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).
Synonyms	Neuromodulin
Reference Data

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