Cytokeratin 4 (KRT4) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunoblotting: 1/3000 for Western blotting (ECL). Cytological material. Immunofluorescence on Frozen Sections: 1/200. Immunohistochemistry on Paraffin Sections: 1/100 after microwave treatment. Incubation Time: 1h at RT, extended with paraffin sections. Positive Control: Squamous epithelium of esophagus, gingiva, palate, vagina (staining all suprabasal layers).
Reactivity	Human, Mouse
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide (C-KIISTTTLNKRR) of Human keratin K4 coupled to KLH
Specificity	This antibody recognizes Human type II (basic) keratin K4 (Mr 59 000), formerly also designated cytokeratin 4. This antibody is an excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin. Recognizes keratin K4 in non-cornifying squamous epithelia. Keratin K4 is mostly present in combination with keratin K13. In contrast to keratin K13, the superficial cells of the cornea are reactive with keratin K4. The antibody also stains ciliated pseudo-stratified epithelium of bronchi and, focally, ductal epithelial cells.
Formulation	State: Serum State: Liquid Stabilized Antiserum Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	keratin 4
Database Link	Entrez Gene 3851 Human UniProt ID P19013
Background	Intermediate filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprised of at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 4 is a member of the type II, neutralto- basic subfamily. It is a 59 kD polypeptide differentially expressed in various human tissues. Cytokeratin 4 can be detected by biochemical or immunohistochemical means in adult non-cornified squamous epithelium such as that of the superficial and intermediate cells of the esophagus, exocervix, tongue, vagina, larynx,pharynx, epiglottis, and anus as well as in the superficial cells of the cornea. In addition, its presence was demonstrated in suprabasal cells of the urinary bladder transitional epithelium, in single cells and cell groups of sweat glands and prostatic ducts and in cylindrical ciliated bronchial cells. Cytokeratin 4 may be detected in squamous cell carcinomas derived from several non-cornified stratified epithelia.
Synonyms	KRT4, CYK4, Cytokeratin-4, CK4, Keratin-4, K4
Reference Data

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