Desmin (DES) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting: 1/100–1/500. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Sections. Rrecommended Dilutions: 1/25-1/200 for immunohistochemistry with avidin biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Reactivity	Bovine, Canine, Chicken, Goat, Hamster, Human, Mouse, Rat, Zebrafish
Host	Rabbit
Clonality	Polyclonal
Immunogen	Chicken gizzard muscle Desmin. Desmin was purified from a crude tissue preparation by preparative gel electrophoresis as described in Reference 1.
Specificity	This Desmin K5 polyclonal antibody reacts exclusively with Desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.
Formulation	State: Serum State: Liquid Serum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	desmin
Database Link	Entrez Gene 1674 Human UniProt ID P17661
Background	Desmin (53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
Synonyms	DES
Reference Data

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