HIF3 alpha (HIF3A) (N-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/128000 (Detection Limit). Immunohistochemistry on Paraffin Sections: 4-6 µg/ml. In paraffin embedded Human Kidney shows nuclear staining in some of the DCT.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the N-Terminus of Human HIF3A (NP_690007.1, NP_690008.2)
Specificity	This antibody is expected to recognize Isoform a and c of HIF3A (NP_690007.1, NP_690008.2).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	hypoxia inducible factor 3 alpha subunit
Database Link	Entrez Gene 64344 Human UniProt ID Q9Y2N7
Background	One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits (HIF1-a, HIF2-a, or HIF3-a) bound to the aryl hydrocarbon receptor nuclear translocator (ARNT) that is also known as HIF1-b. HIF-a is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis . Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.
Synonyms	HIF-3 alpha, Hypoxia-inducible factor 3 alpha, BHLHE17, MOP7, PASD7, Member of PAS protein 7, IPAS
Reference Data

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