THBS3 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to NP_009043.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide.  Immunohistochemistry on Paraffin Sections: 1-2 µg/ml. In paraffin embedded Human Colon shows endothelial cell staining of a blood vessel.
Reactivity	Bovine, Canine, Human, Mouse, Porcine
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C-Terminus of the protein sequence according to NP_009043.1.
Specificity	This antibody recognizes THBS3.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	thrombospondin 3
Database Link	Entrez Gene 7059 Human UniProt ID P49746
Synonyms	TSP3
Reference Data

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