TNF alpha (TNF) Goat Polyclonal Antibody

CAT#: AP33336BT-N

TNF alpha (TNF) goat polyclonal antibody, Biotin

Size: 25 ug 50 ug

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 50 ug

Product images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommend Dilution Direct ELISA: To detect Human TNF-α by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha.
Sandwich ELISA:
To detect Human TNF-alpha by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Anti-Human TNF-alpha (Cat.-No AP33336PU) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha.
Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Goat
Clonality Polyclonal
Immunogen Highly pure (> 98%) E.coli derived recombinant Human TNF-alpha (Cat.-No PA132)
Specificity This antibody recognizes Human TNF-alpha. Other species not tested.
Formulation PBS, pH 7.2
Label: Biotin
State: Lyophilized purified Ig fraction
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography
Conjugation Biotin
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Gene Name tumor necrosis factor
Synonyms TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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