TNF alpha (TNF) Goat Polyclonal Antibody
CAT#: AP33336BT-N
TNF alpha (TNF) goat polyclonal antibody, Biotin
Size: 25 ug
Conjugation: Unconjugated
Need it in bulk or conjugated?
Get a free quote
CNY 4,790.00
Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommend Dilution | Direct ELISA: To detect Human TNF-α by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Sandwich ELISA: To detect Human TNF-alpha by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Anti-Human TNF-alpha (Cat.-No AP33336PU) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98%) E.coli derived recombinant Human TNF-alpha (Cat.-No PA132) |
Specificity | This antibody recognizes Human TNF-alpha. Other species not tested. |
Formulation | PBS, pH 7.2 Label: Biotin State: Lyophilized purified Ig fraction |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography |
Conjugation | Biotin |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Gene Name | tumor necrosis factor |
Database Link | |
Synonyms | TNF, TNF-a, TNFA, TNFSF2, Cachectin |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |