GFP Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1-2 µg/ml.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant GFP protein. The anti-GFP antibody was generated by immunization of rabbits with highly purified recombinant GFP protein produced in E. coli.
Specificity	The antibody was made against GFP from the jellyfish Aequorea Victoria using the whole native protein as an antigen.
Formulation	PBS, pH 7.4, without preservatives State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P42212
Background	Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
Synonyms	Green fluorescent protein, GFP-Tag
Reference Data

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